KMID : 1161420170200040392
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Journal of Medicinal Food 2017 Volume.20 No. 4 p.392 ~ p.402
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Cerebrosides from Sea Cucumber Protect Against Oxidative Stress in SAMP8 Mice and PC12 Cells
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Che Hongxia
Du Lei Cong Peixu Tao Suyuan Ding Ning Wu Fengjuan Xue Changhu Xu Jie Wang Yuming
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Abstract
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Alzheimer's disease (AD) is a neurodegenerative disorder. Emerging evidence implicates ¥â-amyloid (A¥â) plays a critical role in the progression of AD. In this study, we investigated the protective effect of cerebrosides obtained from sea cucumber against senescence-accelerated mouse prone 8 (SAMP8) mice in vivo. We also studied the effect of cerebrosides on A¥â-induced cytotoxicity on the rat pheochromocytoma cell (PC12) and the underlying molecular mechanisms. Cerebrosides ameliorated learning and memory deficits and the A¥â accumulation in demented mice, decreased the content of malondialdehyde (MDA), 8-oxo-7,8-dihydro-2¡Ç-deoxyguanosine (8-OHdG), 8-hydroxy-2¡Ç-deoxyguanosine (8-oxo-G), and nitric oxide (NO), and enhanced the superoxide dismutase (SOD) activity significantly. The neuroprotective effect of sea cucumber cerebrosides (SCC) was also verified in vitro: the cerebrosides increased the survival rate of PC12 cells, recovered the cellular morphology, downregulated the protein levels of Caspase-9, cleaved Caspase-3, total Caspase-3, and Bax, and upregulated the protein level of Bcl-2, revealing that cerebrosides could inhibit A¥â-induced cell apoptosis. The results showed the protective effect of SCC was regulated by the mitochondria-dependent apoptotic pathway. Our results provide a new approach to developing the marine organisms as functional foods for neuroprotection.
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KEYWORD
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Alzheimer's disease, ¥â-amyloid, cerebrosides, neuroprotection, SAMP8, sea cucumber
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